ndrg1-ot1 (264–392)  (Addgene inc)

Journal: RNA Biology

Article Title: Different effects of long noncoding RNA NDRG1-OT1 fragments on NDRG1 transcription in breast cancer cells under hypoxia

doi: 10.1080/15476286.2018.1553480

Figure Lengend Snippet: The primers for quantitative RT-PCR.

Article Snippet: After seeding overnight, cells were co-transfected with 40 μg of NDRG1-OT1 (264–392 bp) and HIF-1α P402A/P564A-pcDNA3 (Addgene, USA), a HIF-1α mutant resistant to PHD2-mediated hydroxylation and VHL-mediated ubiquitination/degradation, for 24 h. Next, total RNA was extracted by RNA immunoprecipitation (RIP) lysis buffer containing a protease inhibitor cocktail.

Techniques: Sequencing

Journal: RNA Biology

Article Title: Different effects of long noncoding RNA NDRG1-OT1 fragments on NDRG1 transcription in breast cancer cells under hypoxia

doi: 10.1080/15476286.2018.1553480

Figure Lengend Snippet: The primers for quantitative RT-PCR.

Article Snippet: After seeding overnight, cells were co-transfected with 40 μg of NDRG1-OT1 (264–392 bp) and HIF-1α P402A/P564A-pcDNA3 (Addgene, USA), a HIF-1α mutant resistant to PHD2-mediated hydroxylation and VHL-mediated ubiquitination/degradation, for 24 h. Next, total RNA was extracted by RNA immunoprecipitation (RIP) lysis buffer containing a protease inhibitor cocktail.

Techniques: Sequencing

Journal: RNA Biology

Article Title: Different effects of long noncoding RNA NDRG1-OT1 fragments on NDRG1 transcription in breast cancer cells under hypoxia

doi: 10.1080/15476286.2018.1553480

Figure Lengend Snippet: The primers for quantitative RT-PCR.

Article Snippet: Plasmid DNA construction and transfection To construct different fragments of plasmid pcDNA3.1 + - NDRG1-OT1 , the nucleotide (nt) fragments 1–149, 150–263, 1–263, and 264–508, cut by Bam H1 (New England BioLabs, Ipswich, MA, USA) and Eco R1 (New England BioLabs) from plasmid pcDNA3.1 + - NDRG1-OT1 , were inserted into pcDNA3.1 + plasmid (Addgene, USA).

Techniques: Sequencing